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PMMH’s weekly seminar is held every Friday at 11 am (map)
Stéphane Perrard
Etienne Reyssat
Virgile Thiévenaz
PMMH
BARRE CASSAN
BAT A 1ER ETAGE CASE 18
7 QUAI SAINT BERNARD
75005 PARIS
France
Tel : (33) 1 40 79 45 22
Séminaire PMMH – Irène Nagle (TU Delft)
Mechanics of collagen networks with active cellular inclusions
Tissues are composed of extracellular matrix (ECM) and cells that are in constant interplay. While ECM influences many cellular processes from division and migration to differentiation, cells interact with their surrounding environment by exerting pulling forces, and remodelling or degrading the matrix, thus altering its structural and rheological properties. How these local matrix changes originated by the cells affect the overall global mechanics of tissues is not fully understood yet. Furthermore, a clear disentangling between the contributions of passive mechanical effects versus active cellular effects to tissue mechanics is still lacking. Here, we address this question by investigating how local cell-matrix interactions regulate the global mechanics of collagen networks in a biomimetic tissue model composed of fibrillar collagen type I with embedded invasive cancer cells (MDA-MB-231). We find by bulk shear rheology that above a cell volume fraction of only a few percents, the mechanics of the collagen network is strongly affected. We observe a softening both of the mature network and over time during collagen polymerization, as well as a loss of the classic stress-stiffening behaviour of collagen. We decouple passive and active contributions by using cell-sized passive inclusions, blocking cell adhesion and contractility, and through rheo-confocal microscopy for simultaneous rheology measurements and in situ visualisation. Our findings bring insights into how active inclusions alter the mechanics of fibrous networks, contributing to a better understanding of the role of cells in the mechanics of healthy but also diseased tissues.
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Seminars (1)
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Séminaire PMMH – Morgan Chabanon
Du 2025 à 11h00 au 0001 à 00h00 - Salle réunion PMMH 1
The audience is composed of people with rather heterogeneous backgrounds including specialists in solids, fluids, granular flows, statistical physics... so the idea is to keep your talk understandable by people not necessarily working in your field... The seminar time slot runs from 11am to noon so the best is to make the talk last around 45 minutes to leave some time for discussion.
- Séminaires ESPCI-ENS de biophysique
- Séminaires du Département de Physique de l’ENS
- Séminaires du Laboratoire d’Hydrodynamique de l’X
- Séminaire de Mécanique d’Orsay (page web FAST)
- Séminaire de Mécanique d’Orsay (page web LIMSI)
- Séminaire de Mécanique des Fluides de l’Institut Jean le Rond d’Alembert
- Séminaires du laboratoire MSC, Paris VII
- Séminaires Gulliver
Link to cofee seminar (internal, every Thursday)
